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SNP Analysis of the Growth Hormone Gene in Indigenous Philippine Cattle, Ilocos Genetic Group by PCR-RFLP
Polymorphism in the bovine growth hormone (GH) gene was detected in indigenous Philippine cattle,Ilocos genetic group using PCR-RFLP. There has been an association made between the GH gene and growth. A SNP site at the position 127 of the amino acid chain has been associated to variability in economically important traits. Digestion of the PCR products of the GH gene with the restriction enzyme AluI showed alleles L and V. The most frequent genotype was LV. The frequency of the L allele was 0.43 while V allele was 0.57. The number of genotypes and allele frequencies for this polymorphism of GH gene so that three patterns were observed and frequencies were 0.20 (n=11), 0.45 (n=25), and 0.35 (n=19) for LL, LV, and VV genotypes, respectively. Based on the observed and expected genotype frequencies the population was in Hardy-Weinberg equilibrium.
Keywords:
Cattle
Growth Hormone Gene
Polymorphism
PCR-RFLP
