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Microarray analysis indicates that vitamin A alters expression profiles of bovine intramuscular preadipocytes during adipogenesis
Beef marbling is an economically important trait in the beef industry. Vitamin A (VA) is an important feed supplement for cattle, but in excess it could hinder marbling. In cattle, VA forms various derivatives such as all-trans retinoic acid (all-trans RA) and 9-cis retinoic acid (9-cis RA). So, in this study, we investigated the genes involved in bovine intramuscular adipogenesis after VA treatment with all-trans RA and 9-cis RA. Differential gene expression levels were validated by microarray analysis in a clonal bovine intramuscular preadipocyte (BIP) cell line. The all-trans RA and 9-cis RA treated cells had reduced transcription of genes involved in the circulatory system and muscle development compared with no RA treatment. The major conclusion from these results is that the genes of muscle development controlled by vitamin A are related to adipogenesis in BIP.
Keywords: bovine, adipogenesis, retinoic acid
