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RAD Sequencing reveals genome-wide heterozygote deficiency in pair crosses of the Chilean mussel Mytilus spp
Mussels are increasingly important aquaculture species. Selective breeding utilizing genomic resources is at a formative stage. We develop genome-wide markers and investigate their inheritance in two Chilean mussel families using a restriction-site associated DNA (RAD) sequencing approach. A total of 4,537 SNP markers were discovered. RAD sequence analysis indicated that mussels are among the most polymorphic species studied to date, with an average frequency of 1 SNP each 25bp. Family-based sequencing allowed us to examine the inheritance pattern of markers. The majority of SNPs (72%) deviate from Mendelian segregation ratios, showing a trend towards a deficiency of heterozygote genotypes in the offspring. Additionally, many alleles were observed in the mussel offspring without being present in their parents. Our results indicate that the inheritance of markers has yet to be fully understood before they can be applied for the genetic improvement of mussels.
Keywords:
Chilean mussel
Heterozygote deficiency
RAD-Sequencing
