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Comparative Study of 13 Candidate Genes Applying Multi-reference Normalization to Detect the Expression of Different Fineness in Skin Tissues of Wool Sheep

Monday, August 18, 2014
Posters (The Westin Bayshore)
Yuezhen Tian , College of Animal Science, Xinjiang Agricultural University, Urumqi, China
Xixia Huang , College of Animal Science, Xinjiang Agricultural University, Urumqi, China
Jiang Di , Xinjiang Academy of Animal Science, Urumqi, China
Kechuan Tian , Xinjiang Academy of Animal Science, Urumqi, China
Weiwei Wu , Xinjiang Academy of Animal Science, Urumqi, China
Xinming Xu , Xinjiang Academy of Animal Science, Urumqi, China
Yanhua Zhang , Xinjiang Academy of Animal Science, Urumqi, China
Hanikezi Tulafu , Xinjiang Academy of Animal Science, Urumqi, China
Abstract Text:

This study aimed at finding some novel molecular markers associated with wool traits and providing  molecular bases for improved wool quality of sheep. Comparative study of 13 candidate genes was designed to evaluate the different fineness in skin tissue of wool sheep applying 18SrRNA,β-Actin and GAPDH as multi-reference normalization and to detect their expression. The results indicated that significant differences were observed in TXNIP, PIK3CA, TFDP1, ADAM9, FAIM and FZD3 expression in different fineness of wool in the skin tissues of sheep.  Comparing the type of wool fineness in sheep, the expression levels of TXNIP, TFDP1 and FAIM gene were higher in the skin tissues of superfine type wool sheep, the expression levels of PIK3CA, ADAM9 and FZD3 genes were higher in the skin tissues of fine type wool sheep. The rest of 7 candidate genes showed no significant difference between the two types.

 Keywords:

wool sheep

candidate genes

multi-reference

See more of: Posters: Prediction using molecular information (Group 3)
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