Sequence Analyses of Bovine PAG-1 Gene

Abstract Text: PAG-1 gene has been identified as prenatal marker for pregnancy diagnosis in cattle. This study was undertaken to characterize and analyse it. Nucleotide and amino sequences of PAG-1 in cattle, other PAG gene family members and some species were retrieved from GenBank and UniProt database and used. Percent identity and similarity between bovine PAG-1 and PAG-1 genes of other species ranged from 29-83% and 44-87%, respectively. The highest sequence identity (83%) and similarity (90%) was observed between bovine PAG-1 gene and bovine PAG-16 gene. Prediction of protein structure of cattle PAG-1 gene revealed that the protein contained alpha helix, extended strand and random coil. Phylogenetic tree constructed between cattle PAG-1 gene, PAG-1 gene of other species and PAG family members showed that PAG-1 gene was closely related to some family members. It was observed that PAG-1 protein was stable, hydrophilic and extracellular.

Keywords: PAG-1 Gene, Phylogenetics, Cattle