268
The Development and Characterization of a 57K SNP Chip for Rainbow Trout

Tuesday, August 19, 2014: 2:30 PM
Cypress Room (The Westin Bayshore)
Yniv Palti , NCCCWA-ARS-USDA, Kearneysville, WV
Guangtu Gao , NCCCWA-ARS-USDA, Kearneysville, WV
Thomas Moen , Aquagen, Aas, Norway
Sixin Liu , NCCCWA-ARS-USDA, Kearneysville, WV
Matthew P Kent , Centre of Integrative Genetics, Aas, Norway
Sigbjorn Lien , Centre of Integrative Genetics, Aas, Norway
Michael R Miller , UC Davis, Davis, CA
Caird E Rexroad , NCCCWA-ARS-USDA, KEARNEYSVILLE, WV
Abstract Text: :  In this paper we describe the development and characterization of the first high density SNP chip for rainbow trout.  The SNPs are distributed throughout the genome with good representation in all 29 chromosomes.  The genotyping quality was high and validation rate was close to 90%.  This is comparable to other farm animals and is much higher than previous smaller scale SNP validation studies in rainbow trout.  The chip is more useful for rainbow trout aquaculture populations with more than 83% polymorphic markers per population, but even in wild populations the number of polymorphic markers was greater than 10,000.  The SNP chip is publically available and it has already been used in a proof- of concept study to demonstrate its utility for genome enabled selection in rainbow trout presented in a companion WCGALP 2014 paper by Vallejo et al. 

Keywords:

Rainbow trout

SNP Chip

Genetic diversity