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Expression of TLR2 Pattern Recognition Receptor on Mononuclear Cells of Dairy Cattle Ranked Using Estimated Breeding Values (EBV) of Adaptive Immune Response Traits

Thursday, August 21, 2014
Posters (The Westin Bayshore)
Lauraine C. Wagter-Lesperance , University of Guelph, Guelph, ON, Canada
Douglas C Hodgins , Dept Pathobiology, University of Guelph, Guelph, ON, Canada
Mehdi Emam , University of Guelph, Guelph, ON, Canada
Marlene A Paibomesai , University of Guelph, Guelph, ON, Canada
Bonnie Mallard , Center for Genetic Improvement of Livestock, University of Guelph, Guelph, ON, Canada
Abstract Text:

Toll-like receptor 2 (TLR2) is a pattern recognition receptor expressed on the surface of epithelial cells and some leukocytes.  Binding of bacterial ligands such as lipoproteins and lipopeptides to TLR2 induces proinflammatory innate responses and contributes to the development of adaptive immune responses. In the present study we investigate whether expression of TLR2 by blood mononuclear cells differs among Holstein dairy cows classified as high (H), average (A), and low (L) immune responders, based on their EBVs for antibody-mediated (AMIR) and cell-mediated (CMIR) immune responses. Cells were stained using fluorochrome-labeled monoclonal antibodies, and characterized using flow cytometry. A significantly higher proportion of unstimulated mononuclear cells from H-AMIR cows expressed TLR2, compared to cells from L-AMIR, and H-CMIR cows. Expression of TLR2 may contribute to the enhanced antibody responses and reduced incidence of clinical disease reported for H-AMIR cows.

Keywords:

dairy cattle

TLR2

innate

See more of: Posters: Genetics of Trait Complexes - Disease Resistance (Group 3)
See more of: Genetics of Trait Complexes: Disease resistance
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