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Use of DNA Markers in Parentage Testing in Mexican Jersey Cattle
The objective was to study the effectiveness of an 11 molecular panel for paternity testing in Mexican Jersey cattle. Seventy blood samples were collected from four sires, 32 dams, and 34 progeny. Commercial kit Maxwell 16 LEV blood AS1010 was used to extract DNA. Microsatellites were amplified using StockMarks for Cattle® Bovine Genotyping Kit. Results were read and analyzed using GeneMapper 4.0 and Cervus 3.0 software. The 11 microsatellites had: high polymorphic information content (0.66), combined probability of exclusion (CPE) of 0.9957, CPE for one candidate parent of 0.9917, CPE for one candidate parent given the genotype of a known parent of the opposite sex of 0.9997, average number of alleles per locus of 8.73, and frequency of the most common allele varied from 0.29 to 0.86. The 11 microsatellite panel evaluated yields enough information to test paternity in Jersey dairy cattle.
Keywords:
Jersey cattle
Parentage verification
DNA
