Transcriptome comparison between the pubertal and adult testis in goat

Abstract Text: Reproduction is essential to goat and any other animal production. Understanding the molecular mechanism in male goat testis development and sperm formation will provide new insights in improving male reproduction. The objective of this study was to carry out a transcriptome comparison between two different development stages of testes, i.e.before and after sexual maturation, in goat using a RNA-seq approach. We generated approximately 50 million reads (75 bp/read) for each testis samples, and identified 5512 and 5216 genes/tranascripts in the pubertal and adult testis, respectively. Our preliminary analysis identified a total of 796 differentially expressed genes between the two different development stages of testes, which were significantly enriched with GO terms related to testis development and male functions.

Keywords: RNA-seq, VLDL, testis transcriptome, cashmere goat,