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The First Whole Transcriptome Profiling of Fibro/adipogenic Progenitor Cells and Discovery of Essential Genes Potentially Favorable to either Myogenesis or Adipogenesis in Muscle

Monday, August 18, 2014: 4:45 PM
Bayshore Grand Ballroom A (The Westin Bayshore)
Rui Li , Washington State University, Pullman, WA
Shanker Dayal , ICAR Research Complex for Eastern Region, Bihar, India
Xiang Zhou , Washington State University, Pullman, WA
Xing Fu , Washington State University, Pullman, WA
Jennifer J Michal , Washington State University, Pullman, WA
Waqas Khan , University of Sargodha, Sargodha, Pakistan
Min Du , Washington State University, Pullman, WA
Zhihua Jiang , Washington State University, Pullman, WA
Recorded Presentation (flv)
Abstract Text:

Wagyu cattle are well known for producing beef with superior marbling. In general, Wagyu cattle accumulate more intramuscular fat in carcass as compared to Angus cattle. Therefore, fibro/adipogenic progenitor cells derived from both breeds should present extremely valuable resources for unraveling molecular mechanisms involved in muscle adipogenesis.  Using the digital gene expression tag profiling (DGETP) method we found that there are at least 8,038 genes expressed in bovine fibro/adipogenic progenitor cells.  Among them, 7,357 genes were assigned to relevant GO (gene ontology) terms. By careful examination of our experimental data using cells from three Wagyu and three Angus, we discovered only 32 genes that were differentially expressed between these two breeds.  Understanding these genes and their functions would help develop strategies to enhance marbling and reduce connective tissue in beef.

Keywords:

Transcriptome profiling

Fibro/adipogenic progenitor cells

Gene ontology

Differentially expressed genes

Beef marbling and connective tissues

See more of: Genetics of Trait Complexes: Growth and Development
See more of: Genetics of Trait Complexes: Growth and development
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