This is a draft schedule. Presentation dates, times and locations may be subject to change.

407
Effects of Bacillus-Based Direct-Fed Microbials on Growth and Gut Health of Nursery Pigs

Tuesday, July 11, 2017: 3:15 PM
317 (Baltimore Convention Center)
Kayla L. Brooks, North Carolina State University, Raleigh, NC
Sung Woo Kim, North Carolina State University, Raleigh, NC
The objective of this study was to determine the effects of Bacillus-based direct-fed microbials (DFM) on growth performance, gut health, immune status, and oxidative stress status in nursery pigs. Pigs (180 barrows and 180 gilts) at 21 d of age and 6.26 ± 1.02 kg BW were randomly assigned to 3 treatments based on a randomized complete block design with sex and initial BW as blocks: 1) Basal diet (CON); 2) Basal diet + DFM based on 1 Bacillus species (3.34 x 109 CFU/kg feed) (DFM-1); 3) Basal diet + DFM based on 2 Bacillus species (1.28 x 109 CFU/kg feed) (DFM-2). Pigs were distributed into 30 pens per treatment with 4 pigs per pen. Pen was the experimental unit and proc MIXED of SAS was used for statistical analysis using treatment and sex as fixed effects and BW block as a random effect. Pigs were given ad libitum access to feed and water for 42 d with d 1 to 14 representing phase 1 and d 15 to 42 representing phase 2. Feed intake and BW were measured at the beginning and end of each phase to calculate ADG, ADFI, and G:F. At d 42, 8 pigs (1 per pen) per treatment (4 barrows and 4 gilts) were euthanized for sample collection. Serum and jejunal mucosal tissues were obtained to measure tumor necrosis factor α (TNF-α), immunoglobulins G and A (IgG and IgA), protein carbonyl (PC) and malondialdehyde (MDA). Jejunal tissues were obtained to measure tight junction proteins (claudin, occludin, and ZO-1). The ADG was greater (P < 0.05) in the DFM-1 (550 and 427 g/d) and DFM-2 (546 and 424 g/d) compared to the CON (503 and 392 g/d) for both phase 2 and the overall experimental period, respectively. Overall ADFI was increased (P < 0.05) in the DFM-1 (552 g/d) and DFM-2 (555 g/d) compared to the CON (516 g/d), although there were no effects on G:F. The DFM-1 had higher (P < 0.05) ZO-1 than the CON. The DFM-1 had higher (P < 0.05) claudin and ZO-1 than the DFM-2. There were no differences in TNF-α, IgG, IgA, PC, or MDA concentrations in serum and jejunal mucosa among treatments. In conclusion, both Bacillus-based DFMs improved growth of nursery pigs primarily by increasing feed intake whereas the DFM with 1 Bacillus strain additionally improved gut integrity without affecting immune or oxidative stress status in the small intestine.