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Vitamin C Induces PRDM16 of Bovine Muscle-Derived Cell Isolated from Semimembranosus and Longissimus Dorsi Tissues
Vitamin C Induces PRDM16 of Bovine Muscle-Derived Cell Isolated from Semimembranosus and Longissimus Dorsi Tissues
Monday, July 10, 2017
Exhibit Hall (Baltimore Convention Center)
Vitamin C (VC) supplements have been used for enhancing marbling fat in high quality beef cattle. However, mode of action of vitamin C was not clearly studied for a long time. The aim of this experiment was to determine the effect of VC dose titration in bovine muscle-derived cell (MDC) from semimembranosus (SM) and longissimus dorsi (LD) muscle tissues. We hypothesized that VC may affect adipogenesis of bovine MDC isolated from different muscle depots. Bovine MDC were pronase-liberated from SM and LD muscle tissues of three new-born Hanwoo calves. Bovine SM and LD MDC were incubated with dulbecco modified eagle medium (DMEM) with 10% fetal bovine serum for proliferation and induced differentiation with DMEM with 3% horse serum. Bovine MDC were treated with various levels of VC (control, 10, and 100μM) with insulin, oleic acid, and ciglitizone (IOC) to induce transdifferentiation in bovine MDC cultures. Relative mRNA abundance for adipogenic markers (PRDM16, C/EBPβ, PPARγ, SCD, GPR43) and myogenic markers (Pax3, Myf5, myogenin, GLUT4, MHCI, MHC IIX) were analyzed with Real-Time quantitative PCR for 2, 3, and 7 days after differentiation treatment. Data were analyzed as a completely randomized design using the MIXED model, each treatment performed in triplicate. Means were considered different at P<0.05. Relative PRDM16 mRNA abundance was increased in dose-dependent levels in both SM and LD MDC with VC treatments compared to the control (P<0.05). The levels of C/EBPβ and PPARγ were increased during differentiation (P<0.05) but level of SCD was not changed during differentiation (P>0.05). The levels of Pax3, Myf5 and myogenin were increased with treatment of 100μM VC but MHC IIX was only greater at 100μM VC in SM MDC than those in LD MDC (P<0.05). There was not a tissue x time interaction (P>0.05) but there were main effects (P<0.05) among adipogenic and myogenic mRNA concentration. These results indicated that VC have a dose-dependent effect on not only PRDM16 but also on adipogenic markers in bovine MDC isolated from LD and SM muscle tissues.