This is a draft schedule. Presentation dates, times and locations may be subject to change.

565
Effects of Heat Load and Active Dry Yeast Supplementation on Ruminal Parameters

Monday, July 10, 2017: 3:30 PM
310 (Baltimore Convention Center)
Whitney L Crossland, Texas A&M University, College Station, TX
Aaron B. Norris, Texas A&M University, College Station, TX
Todd R. Callaway, USDA-ARS, College Station, TX
Luis O. Tedeschi, Texas A&M University, College Station, TX

An active dry yeast (ADY) supplement (Vistacell, AB Vista, UK) was proposed to favorably influence ruminal parameters of heat loaded cattle. Eight British-cross steers (365 ± 27 kg) were fed a 90 % concentrate diet (1.8 % of BW intake, DM basis) and supplemented with either ADY (3.0 g/d; providing 6 × 1010 CFU/d) or no yeast (0.0 g/d; CON) for 14 d. Rumen boli were inserted through the esophagus on d 0 for continuous monitoring of pH and rumen temperature. On d 13 and 14, steers were housed in an open circuit calorimetry chamber for 48 h in either thermoneutral (TN) (18 ± 0.55°C; 35% RH) or heat loaded (HL) (35 ± 0.55°C; 35% RH) temperature. After 48 h, rumen fluid (500 mL) was extracted via esophageal pump and immediately processed for further analysis and in vitro fermentation using a 2×2 factorial arrangement with the in vitro gas production technique (IVGP). The IVGP revealed an effect of ADY on DMD versus CON (61.7 % vs. 32.8 %, P = 0.031) but there was no effect of temperature. No effects were observed on the fermentation dynamics of the DM. There was a significant interaction of supplement and temperature on estimated ME in which ADY+TN, CON+HS, and ADY+HL were greater (P = 0.0307) than CON+TN (2.97, 2.84, 2.72 vs. 1.95 Mcal/kg, respectively). The IVGP of methane (CH4) g/L or CH4 g/L/g of DM digested was not affected. Mean rumen pH of steers tended to be affected by temperature and supplement: HL steers had lower pH than TN (P = 0.064), and CON steers had lower pH than ADY (P = 0.077). Time spent below pH of 6 was affected by supplement: ADY was much less than CON (390.0 vs. 882.5 min/d, P = 0.05). Time spent below pH of 5.0 was affected by temperature: TN was much less than HL (1.75 vs. 590.75 min/d, P = 0.034, respectively). Mean rumen temperature and time spent above 40°C was affected by temperature but not by supplement. As expected HL steers had greater rumen temperature and spent more time above 40°C than TN steers (40.51 vs. 39.21 °C, P = 0.0165; 1,216.25 vs. 27.5 min/d, P = 0.002). Our results indicated that supplementation of 3.0 g/d of an ADY in the diets of steers consuming 90 % concentrate diets under severe heat loads may increase DMD, increase available ME, and relieve subacute ruminal acidosis.