This is a draft schedule. Presentation dates, times and locations may be subject to change.
874
Effect of Dietary Fish Oil and Vitamin E on DNA Damage in Dogs Undergoing Training
Using a replicate 3x3 Latin square design, six male dogs (2-6 yrs, 21-35 kg) were assigned to three groups: i. control diet (CG), ii. Same diet supplemented with 54 mg FO/kg body weight0.75 (FG), and iii. similar diet plus FO (54 mg//kg body weight0.75) and 400 mg VE (FEG) per day for 60 days. Blood samples were collected on days 0, 30 and 60. DNA damage was measured using a single cell gel electrophoresis assay (SCEG) in peripheral whole blood leukocytes. DNA damage was classified in four classes (I, undamaged; II, minimum damage; III, medium damage; IV, maximum damage. Furthermore, a DNA damage index (DI) was calculated using the formula DI = [(I) + 2(II) + 3(III) + 4(IV)]/N(0--IV), where 0--IV represent the nucleoid type, and N0--NIV represent the total number of nucleoids scored. Dogs were trained on a treadmill at 8 km/h and a 7.5% slope twice a week for 60 days. Each session lasted 30 min. Data were analyzed using the Proc mixed procedure of SAS (9.4). Treatment, time and their interaction were considered fixed variables and dog and period random. Slice option of SAS was used for mean separation when there was a treatment by time interaction. On day 30 there was a significant increase in DI in FG compared with CG and FEG (P<0.01). DNA damage index values were 0.002, 0.105, 0.109, 0.053, 0.743, 0.467, 0.175, 0.127, 0.193 for CG, FG VEG on day 0, 30 and 60, respectively. There was a time by treatment interaction for the serum VE concentration (P<0.01). On day 0 and on day 30 there were no differences in serum VE concentrations of the three groups (P>0.1). However, on day 60 serum VE concentrations were higher in FEG compared with C and FG (6200 vs. 2300 vs. 2000 µg/d, respectively; P<0.01). In conclusion, FO supplementation produces DNA damage after 30 days, but not at 60. The supplementation of FO with VE could prevent the damage at 30 days. Supplementation with VE increases serum VE concentration.