Kinetics of gas production of soybean meal, cotton seed meal and fish meal is affected using different zeolites
An in vitro experiment was carried out to study the effect of four types of zeolites (Clinoptilolite 1, Clinoptilolite 2, Clinoptilolite A and Heulandites) on kinetics of gas production of three different protein sources (soybean meal, cotton seed meal and fish meal). The cumulative gas production was measured at 2, 6, 10, 14, 18, 22, 24 and 48 hours of incubation using a pressure transducer. Each sample was incubated in three replicates.The incubation inoculum was prepared by diluting the rumen liquor with a buffer solution (Tilley and Terry, 1963). Fifteen millilitres of buffered rumen fluid (20% rumen fluid + 80% buffer solution) prepared and were anaerobically dispensed in each tube at 390 C. All the tubes were crimped, placed in an incubatorat 39 C, and shaken at regular times. A factorial experiment within completely randomized design (CRD) was carried out for data on gas production parameters from different protein sources and difference zeolites. All analyses were done on MSTATC program. The result of in vitro gas production showed that there were significant differences between total gas production and lag time of different protein sources (P<0.05). Total gas production was affected by different zeolites (P<0.05). A significant effect of feed×zeolite was observed for the rate at which gas was produced (c) (P<0.05). The mean value for total gas production, lag time and the rate of gas production were 15.68 (ml/125 mg DM), 0.06 ( %/h) and 0.31 (h), respectively. Fish meal, among the different protein sources and Clinoptilolite 2 among the different zeolites resulted in the highest gas production rate (0.08). Heulandites produced the highest cumulative gas production (17.41ml/125mg DM) as compared to the other zeolites.
Gas production, Zeolites, Protein sources.