Sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica excretory secretary in goats sera.

1. Abstract Text: Bachground: Fascioliasis is considerd a major animals health problem.Many immulogical techniques have been developed over years using the different Fasciola antigens for diagnosis of parasitic infection and to replace the parasitological techniques , which are time consuming and usually proved to be  inadequate and unreliable.Material and methods: Viable F.gigantica fluckes were obtained from infected cows at a local abattoir and kept in suitable meduim inside CO₂ incubator to produce excretory /secretary(E/S) antigenes. The pure E/S was collected and their protein contents were estimated. The bands of sizes 27, 30, 40, 60 and 60/62 were used to  obtain suitable antibody probe to be used in isoloting target antigen of E/S origin from infected Egyptian Nubian goats sera. Results: The 40 kilo Dalton immunogenic of E/S origin sandwich enzyme -linked immunsorbent assay is highly specific in detecting F.gigantica infection in Egyptan Nubian  goats. It also offers a promissing diagnostic tool.

Keywords: Egyptian Nubian goats, Fasciola gigantica , Sandwich