Effect of CALSPORIN® on growth performance, nutrients digestibility, organ weight, meat quality and excreta and intestinal microflora and slurry noxious gas emission in broiler chickens

Abstract Text:

This study was conducted to determine the effect of dietary CALSPORIN^® supplementation on growth performance, nutrient digestibility, relative organ weight, meat quality, intestinal microbial flora, and slurry noxious gas emissions in broilers. A total of 816 1d-old male ROSS 308 broilers (BW of 46.06 g ± 0.67 g; 16 pens/treatment and 17 broilers/pen) were used in a 5-wk study. Broilers were randomly allotted to 1 of 3 dietary treatments and CALSPORIN^® BBS, which contains 1 × 10⁹ CFU/g Bacillus subtilis C-3102.: T1) CON, basal diet, T2) CON + 300 ppm CALSPORIN^® (3 × 10⁵ CFU/g), and T3) CON + 600 ppm CALSPORIN^® (6 × 10⁵ CFU/g) . The T3 enhanced  BW gain (BWG) (700 vs. 658 g; P<0.05) compared with the CON treatment during the d 8-21. During d 22 to 35, T3 showed greater BWG (843 vs. 801 g; Linear, P<0.05) and better feed conversion ratio (FCR) (1.665 vs. 1.792; Linear, P<0.05) than those in CON. The FCR (1.686 vs. 1.792; Linear, P<0.05) in T2 was linearly improved compared with CON. In the overall period, the BWG (1,630, 1,662 vs. 1,573 g; P<0.05) and the FCR (1.544, 1.534 vs. 1.624; P<0.05) in T2 and T3 were higher than the control. The apparent total tract digestibility (ATTD) of dry matter (DM) (73.43 vs. 76.54, 77.20%; P<0.05) and energy was linearly increased (75.34 vs. 78.83, 79.87%; P<0.05) by CALSPORIN^® inclusion in the diet. Slurry NH3 emissions were linearly reduced (9.9 vs. 8.2, 7.8 ppm; P<0.05) by dietary supplementation of CALSPORIN^®. The Lactobacillus counts in cecum and ileal were linearly (6.25，6.59，7.16 log₁₀cfu/g; P<0.05) increased by CALSPORIN^® inclusion in the diet. Dietary supplementation of CALSPORIN^® linearly reduced E. coli counts in cecum (6.12，5.74, 5.43 log₁₀cfu/g; P<0.05) Cl. Perfringens counts in large intestine (3.14, 2.97, 2.86 log₁₀cfu/g; P<0.05) and excreta (3.31, 3.17, 3.03 log₁₀cfu/g; P<0.05), and Salmonella counts in cecum (2.66, 2.23, 2.13 log₁₀cfu/g; P<0.05), ileal (2.87, 2.58, 2.42 log₁₀cfu/g; P<0.05), large intestine (3.04, 2.59, 2.44 log₁₀cfu/g; P<0.05) and excreta (2.88, 2.76, 2.61 log₁₀cfu/g; P<0.05). In conclusion, results indicate that CALSPORIN^® inclusion can improve BWG and FCR, increase cecum Lactobacillus counts and reduce intestinal E. coli, Cl. Perfringens and Salmonella counts, especially at 6 × 10⁵CFU/g.

Keywords: growth performance, probiotics, fecal microbial, broilers