1189
Poor Maternal Nutrition During Gestation Reduces Mesenchymal Stem Cell (MSC) Proliferation in Offspring

Wednesday, July 23, 2014
Exhibit Hall AB (Kansas City Convention Center)
Sambhu M Pillai , Department of Animal Science, University of Connecticut, Storrs, CT
Maria L. Hoffman , Department of Animal Science, University of Connecticut, Storrs, CT
Kristen N. Peck , Department of Animal Science, University of Connecticut, Storrs, CT
Ellen V. Valley , Department of Animal Science, University of Connecticut, Storrs, CT
Thomas D. Crenshaw , University of Wisconsin, Madison, WI
Steven A. Zinn , Department of Animal Science, University of Connecticut, Storrs, CT
Kristen E. Govoni , Department of Animal Science, University of Connecticut, Storrs, CT
Abstract Text:

Poor maternal nutrition may alter bone and adipose development in offspring by diverting MSC from osteoblast to adipocyte lineage. To determine if poor maternal nutrition during gestation alters bone and adipose development and function of MSC in offspring, 36 ewes were fed 100%, 60%, or 140% of requirements (NRC, 1985) beginning at d 31 ± 1.3 of gestation. Lambs from ewes fed 100% (CON), 60% (RES) and 140% (OVER) were euthanized within 24 h of birth (d 1; n = 18) or at 3 mo of age (n = 15). At necropsy, the left tibia and femur were collected for MSC culture and mineral analysis, and backfat thickness and BW were measured. The MSC were isolated from bone marrow at d 1 and cultured (α- MEM + 10% fetal bovine serum + 0.5% penicillin streptomycin + 0.25% Amphotericin-B).  Proliferation of MSC was determined by bromodeoxyuridine assay. Gene expression was quantified using real-time RT-PCR. Data were analyzed using PROC MIXED in SAS. As previously reported, BW were 13% greater in OVER than CON at 1 d and 3 mo (P ≤ 0.05), but not different in RES (P > 0.1). Maternal diet did not affect (P > 0.2) bone length, area, mineral content and mineral density at d 1 or at 3 mo. Backfat thickness was reduced 50% in RES compared with CON at 3 mo (P = 0.01). Compared with CON, MSC proliferation was reduced 51% and 58% in RES (P = 0.07) and OVER (P = 0.03), respectively in the presence of serum and reduced 27% and 44% in RES (P = 0.11) and OVER (P = 0.05), respectively without serum. Expression of markers of MSC commitment to adipocyte and osteoblast cell lineages were evaluated. P2Y purinoceptor 14 was reduced 1.7 ± 0.1 fold in OVER (P = 0.09) and P2Y purinoceptor 1 was reduced 1.7 ± 0.1 fold in OVER (P = 0.09) vs. CON. Whereas C/EBPβ, Msh homeobox 1, Protein delta homolog 1, P2Y purinoceptor 2 were not affected by maternal diet (P > 0.3). Studies are in progress to determine if maternal diet alters differentiation of MSC into osteoblasts and adipocytes. In conclusion, poor maternal nutrition reduces the proliferation of MSC in offspring which may contribute to altered bone and adipose tissue development.

Keywords:  mesenchymal stem cells, proliferation, sheep

See more of: Growth & Development Poster II
See more of: Growth and Development
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