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928
Effect of xylanase and live yeast supplementation on growth performance and gut microflora diversity of growing pigs

Wednesday, July 20, 2016: 9:45 AM
Grand Ballroom F (Salt Palace Convention Center)
Hang Lu , Purdue University, West Lafayette, IN
Hui Yan , Purdue University, West Lafayette, IN
Helen MaseyONeill , AB Vista Feed Ingredients, Marlborough,, United Kingdom
C. L. Bradley , AB Vista, Marlborough, United Kingdom
Mike Bedford , AB Vista Feed Ingredients,, Marlborough, United Kingdom
P. Wilcock , AB Vista, Marlborough, United Kingdom
Cindy Nakatsu , Purdue University, West Lafayette, IN
O. Adeola , Purdue University, West Lafayette, IN
Kolapo M Ajuwon , Department of Animal Sciences, Purdue University, West Lafayette, IN
Abstract Text:

The objectives of current study were to determine the effect of xylanase and live yeast (LY) supplementation, and to determine the timing of xylanase supplementation, on future growth performance and gut microflora diversity of pigs. In the study, 180 weanling pigs (21 ± 2 d, BW: 6.2 ± 0.16 kg) were assigned to 5 treatments in a randomized complete block design (6 replicate pens, 6 pigs per pen) from weaning to market. The 5 treatments were; control-control, control-xylanase, xylanase-xylanase, LY-xylanase, xylanase+LY-xylanase, where the first diet was fed from weaning to 2 wk and thereafter 6 additional corn/soy/corn DDG based phases were fed until d 127. Xylanase (Econase XT) was added at 16,000 BXU/kg and LY (Vistacell) at 1 kg/t. On d 15, 2 pigs per pen were euthanized to obtain jejunal mucosa samples. Fecal samples from days 0, 14, 43 and 127 were collected for volatile fatty acids (VFA) and microbial diversity analysis. Pigs fed with LY and LY+xylanase from d 0-15 had higher BW and ADG at d 15 compared with control (P<0.05). Overall, G:F was higher in the  xylanase+LY-xylanase and control-xylanase groups (P<0.05) on d 127. Additionally, glucose transporter 2 (GLUT2) mRNA expression was higher in the LY and LY+xylanase groups on d 15 compared with control (P<0.05). Furthermore, intestinal alkaline phosphatase (IAP) mRNA expression was highest in the xylanase-xylanase group and lowest in the LY-xylanase group (P<0.05). Fecal VFA concentration significantly increased with age (P<0.01). LY supplementation significantly increased propionate acid, valeric acid and isovaleric acid concentrations on d 127 (P<0.05). Age significantly affected microbial diversity structure (P<0.01). In summary, LY supplementation with or without xylanase improved growth performance of weanling pigs in the first 2 weeks after weaning. Fecal VFA concentrations and microbial community structure were significantly influenced by age, with xylanase and LY supplementation having only a minor effect.

Keywords:  Xylanase, Live yeast, Weanling pigs, Growth, Microbial profile