Addition of optimal non-starch polysaccharides enzymes using in vitro method to a corn-soybean meal diet and a corn-miscellaneous meal diet for growing pigs

Abstract Text:

The objectives of the present study were to evaluate the effect of optimized non-starch polysaccharides (NSP) enzymes using in vitro digestion method on the digestibility of energy and nutrients in a corn-soybean meal diet and a corn-miscellaneous meal diet (corn-soybean meal-rapeseed meal- cottonseed meal- sugar beet pulp meal diet) of pigs, respectively. Exp. 1, the optimal NSP enzymes (cellulase, xylanase, β-glucanase, β-mannanase, α-galactosidase, and pectinase) of the two diets were screened using a quadratic regress-orthogonal rotary design. Exp. 2, the effects of the optimal NSP enzymes on the digestibility of energy and nutrients in the 2 diets were determined. A total of 12 ileal-cannulated pigs (initial BW=50.9±4.9 kg) were allotted to 4 treatments in an incomplete block design (4´3), 4 diets (the 2 diets with or without the addition of optimum NSP enzymes) were fed to the growing pigs. The NSP enzymes had a quadratic effects on the in vitro dry matter digestibility (IVDMD) and the optimal enzymes combination was 534 U/kg of cellulase, 9,984 U/kg of xylanase, 1,014 U/kg of β-glucanase, 4,081 U/kg of β-mannanase, 252 U/kg of α-galactosidase, and 107 U/kg of pectinase in the corn-soybean meal diet (R² = 0.69, P = 0.04), and 960 U/kg of cellulase, 17,178 U/kg of xylanase, 406 U/kg of β-glucanase, 19,023 U/kg of β-mannanase, 307 U/kg of α-galactosidase, and 97 U/kg of pectinase in the corn-miscellaneous meal diet (R² = 0.72, P = 0.02), respectively. Pigs fed the corn-soybean meal diet with the addition of NSP enzymes had greater (P < 0.05) apparent ileal digestibility (AID) of DM, NDF, and insoluble NSP and the apparent total tract digestibility (ATTD) of soluble NSP and total NSP than those fed the diet without NSP enzymes. The AID of all nutrients by the exception of ether extract and the ATTD of GE, DM, CP, EE, and insoluble NSP were greater in a corn-miscellaneous meal diet with the NSP enzymes than without the NSP enzymes (P<0.05). The ATTD of total NSP content had a tendency (P = 0.05) to increase in corn-miscellaneous meal diet with the addition of NSP enzymes. In conclusion, a corn-soybean meal diet and a corn-miscellaneous meal diet with the optimal NSP enzymes using in vitro digestion method can increase nutrient digestibility of diets fed to growing pigs.

Keywords: digestibility, energy, in vitro digestion method, non-starch polysaccharides enzyme, nutrient, pig