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RNA sequencing analysis identifies retinoic acid pathway genes as differentially expressed in animals with extreme intramuscular fat GEBVs in Nellore steers

Friday, August 22, 2014
Posters (The Westin Bayshore)
Aline S. M. Cesar , University of Sao Paulo, Piracicaba, Brazil
Luciana C. A. Regitano , Embrapa Pecuária Sudeste, São Carlos, Brazil
James E. Koltes , Iowa State University, Ames, IA
E. Fritz-Waters , Iowa State University, Ames, IA
Gustavo Gasparin , University of São Paulo/ESALQ, Piracicaba, Brazil
Gerson B Mourao , Department of Animal Science, University of São Paulo/ESALQ, Piracicaba, Brazil
Dante Pazzanese Duarte Lanna , University of Sao Paulo / ESALQ, Piracicaba, Brazil
James M Reecy , Iowa State University, Ames, IA
Luiz L. Coutinho , Universidade de São Paulo/Esalq, Piracicaba, Brazil
Abstract Text:

Intramuscular fat (IMF) quantity is an economically important phenotype, which influences the sensorial and nutritional value of beef. In this study RNA sequencing (RNA-seq) technology was used to identify important genes and pathways related with IMF deposition in Nellore beef cattle. Samples of Longissimus dorsi (LD) muscle from 14 Nellore steers (seven samples with high (H) and seven with low (L) Genomic Estimated Breeding Values (GEBV) for IMF) were used for RNA-seq. A total of 77 DE genes were identified between H and L groups. Pathway analysis revealed important gene networks associated with lipid metabolism, including retinoic acid pathway members: SLC2A4, SPARC, STAT5A and PTAFR.

Keywords:

Beef cattle

Intramuscular fat

Lipid metabolism

Transcriptome

See more of: Posters: Genetics of Trait Complexes: Growth and Development (Group 2)
See more of: Genetics of Trait Complexes: Growth and development
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