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Genetic Bases of Resistance versus Susceptibility to Flavobacterium psychrophilum in Rainbow Trout

Friday, August 22, 2014
Posters (The Westin Bayshore)
Edwige Quillet , INRA, UMR 1313 GABI, Jouy en Josas, France
Nicolas Dechamp , INRA, UMR 1313 GABI, Jouy-en-Josas, France
Caroline Hervet , INRA, UMR 1313 GABI, Jouy-en-Josas, France
Francine Krieg , INRA, UMR 1313 GABI, Jouy-en-Josas, France
Céline Chantry-Darmon , LABOGENA, Jouy-en-Josas, France
Mekki Boussaha , INRA, UMR1313 GABI, Jouy-en-Josas, France
Aurélie Bérard , INRA, US 1279 EPGV (Etude du Polymorphisme des Génomes Végétaux), Evry, France
Véronique Laurens , INSERM UMR 866, Dijon, France
Tatiana Rochat , INRA, UR 892 VIM, Jouy-en-Josas, France
Eric Duchaud , INRA, UR 892 VIM, Jouy-en-Josas, France
Pierre Boudinot , INRA, UR 892 VIM, Jouy en Josas, France
Jean-François Bernardet , INRA, UR 892 VIM, Jouy-en-Josas, France
Christian Michel , INRA, UR 892 VIM, Jouy-en-Josas, France
Abstract Text:

ABSTRACT: Flavobacterium psychrophilum is one of the most significant bacterial pathogen in salmonids worldwide. Selective breeding for resistance seems a promising method to control the disease. We searched for resistance QTL in a doubled haploid F2 progeny from two clonal lines chosen for opposite resistance. About 280 fish (6.3g mean body weight) were infected through intramuscular injection (4.2 105 cfu/fish) and mortality was recorded daily during one month. Using a genome-scan with 217 microsatellite and 101 SNP markers, QTL for survival were detected on several chromosomes, three of them having a strong effect on survival. To get further insight into the defense mechanisms involved, the location of a set of immune candidate genes was determined and the effect of the QTL on bacterial load in the spleen is being investigated.

Keywords:

Bacterial Cold Water Disease

QTL

immune genes

See more of: Posters: Breeding in Aquaculture Species (Group 2)
See more of: Species Breeding: Breeding in aquaculture species
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