Characterisation Of Myofibrillar and Sarcoplasmic Muscle Proteins Of African Catfish: Clarias Gariepinus, Heterobranchus Bidorsalis and Their Reciprocal Hybrids

Abstract Text:

Extracts from sixteen juvenile Clariid fish samples artificially propagated and reared for sixteen weeks were electrophoretically analyzed and separated using 12.5% (SDS-PAGE). The study was aimed to characterize the Clariid species and their hybrids for effective breeding purposes. The relative molecular weight (MW) of individual protein bands were analyzed using TotalLab™ 1D software. The 5^th band with MW of 52.23 KDa of the myofibrilla electrophoregram distinguished C. gariepinus from H. bidorsalis; the 3^rd band with MW 119.04 KDa, 4^th band with MWs 101.49 KDa and 102.13 KDa; 8^th band with MWs 29.24 KDa and 29.39 KDa distinct the pure breeds from the hybrids. However, in sarcoplasmic fraction, the 3^rd and 5^th bands with MWs 92.11 KDa and 54.28 KDa respectively distinguished the hybrids. The 7^th band with MW 41.67 KDa distinct the pure breeds.

Keywords:

Artificial Propagation

Electrophoregram

Juvenile fish