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Monoallelic expression of NNAT gene in Nelore steers skeletal muscle

Monday, August 18, 2014
Posters (The Westin Bayshore)
Marcela M. Souza , Federal University of São Carlos, São Carlos, Brazil
Adhemar Zerlotini , Embrapa Agricultural Informatics, Campinas, Brazil
Polyana C. Tizioto , Federal University of São Carlos, São Carlos, Brazil
Priscila S. N. Oliveira , Federal University of São Carlos, São Carlos, Brazil
Adriana L. Somavilla , Universidade Estadual Paulista "Júlio de Mesquita Filho", Jaboticabal, Brazil
Fabiana B. Mokry , Universidade Federal de São Carlos, São Carlos, Brazil
Aline S. M. Cesar , University of Sao Paulo, Piracicaba, Brazil
Wellison J. S. Diniz , Federal University of São Carlos, São Carlos, Brazil
Maurício A. Mudadu , Embrapa Pecuária Sudeste, São Carlos, Brazil
Simone C. M. Niciura , Embrapa Southeast Livestock, São Carlos, Brazil
Luiz L. Coutinho , Universidade de São Paulo/Esalq, Piracicaba, Brazil
Luciana C. A. Regitano , Embrapa Pecuária Sudeste, São Carlos, Brazil
Abstract Text:

The NNAT gene plays a role on adipogenesis, brain development and insulin secretion. NNAT is described as paternal imprinted in bovine fetal tissue, but it is still underexplored in adult tissues. In order to improve the understanding of NNAT expression, muscle from Nellore was used to assess its allelic expression. Genomic DNA from 38 steers was genotyped for identification of heterozygous individuals with known allele origin. Total mRNA from muscle was extracted and sequenced by HiScanSQ, and afterwards the reads of each allele were counted for each animal. A total of 8 reciprocal heterozygous had more than 10 reads for the chosen SNP. All animals showed monoallelic expression, being 5 with paternal allele expressed, while 7 had the G allele expressed. These results demonstrate that NNAT may not have the paternal imprint conserved in adult bovine muscle, and it suggests a G allele-dependent expression.

Keywords:

Bos indicus

imprint

Neuronatin