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MicroRNAs are involved in bovine mammary gland response to dietary supplementation with safflower oil

Wednesday, August 20, 2014
Posters (The Westin Bayshore)
Ran Li , Agriculture and Agri-Food Canada, Sherbrooke, QC, Canada
Frédéric Beaudoin , Agriculture and Agri-Food Canada, Sherbrooke, QC, Canada
Xin Zhao , McGill University, St Ann De Bell, PQ, Canada
Chuzhao Lei , Northwest A&F University, Xi’an, China
Eveline M Ibeagha-Awemu , Agriculture and Agri-Food Canada, Sherbrooke, QC, Canada
Abstract Text:

The specific roles of microRNAs in the dietary adaption process induced by supplementation of plant oils in the diet of lactating cows are unclear. This study aimed to characterize microRNA expression profiles in bovine mammary glands after supplementation with 5% safflower oil. Nine libraries were constructed from mammary gland biopsies collected from three lactating Holstein cows on Day-14 (control period), 7 and 28 days after onset of treatment with 5% safflower oil and sequenced. Milk fat percentage showed significant(P<0.05) reduction during the treatment period as compared to control period. We identified 322 known microRNAs and 89 novel microRNAs. As compared to the control period, nine microRNAs were up-regulated and six down-regulated at Day+28(P<0.05). Function enrichment of gene targets of differentially expressed microRNAs showed involvement in lipid metabolism(P<0.01), suggesting that these microRNAs could be important regulators of bovine mammary gland lipogenesis.

 Keywords:

microRNA

bovine mammary gland lipogenesis

safflower oil

See more of: Posters: Genetics of Trait Complexes - Lactation
See more of: Genetics of Trait Complexes: Lactation
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