This is a draft schedule. Presentation dates, times and locations may be subject to change.

75
Effects of Poor Maternal Nutrition and Gender on Satellite Cell Metabolism in Lambs

Monday, July 10, 2017
Exhibit Hall (Baltimore Convention Center)
Dominique E. Martin, Department of Animal Science, University of Connecticut, Storrs, CT
Amanda K. Jones, Department of Animal Science, University of Connecticut, Storrs, CT
Sambhu M. Pillai, Department of Animal Science, University of Connecticut, Storrs, CT
Maria L. Hoffman, Department of Animal Science, University of Connecticut, Storrs, CT
Katelyn K. McFadden, Department of Animal Science, University of Connecticut, Storrs, CT
Kristen E. Govoni, Department of Animal Science, University of Connecticut, Storrs, CT
Steven A. Zinn, Department of Animal Science, University of Connecticut, Storrs, CT
Sarah A. Reed, University of Connecticut, Department of Animal Science, Storrs, CT
Poor maternal nutrition during gestation alters offspring muscle composition, mass, fiber number, postnatal growth, and satellite cell function. Satellite cells are quiescent myogenic cells located adjacent to mature muscle fibers that contribute to pre- and post-natal muscle growth. We hypothesized that over- or under-feeding ewes during gestation would negatively impact the metabolic function of muscle satellite cells in the offspring. Pregnant Western White-faced ewes (n = 78) were individually fed 60% (RES), 100% (CON), or 140% (OVER) of NRC requirements for TDN from d 30.2 ± 0.2 of gestation until parturition. Within 24 h of birth, lambs were euthanized for LM collection. Satellite cells were isolated and cultured from the LM of 24 lambs (male: 9, female: 15; n = 8 lambs per diet). The glycolytic and mitochondrial respiration of satellite cells were determined using the Seahorse Bioscience XF analyzer. Proliferative cells were seeded at a density of 7.5x104 in triplicate wells. Respiration data were normalized based on DNA content. Data were analyzed as a completely randomized design using PROC MIXED in SAS with main effects and interaction of gender and maternal diet. There was a main effect of gender for maximal respiration and spare respiratory capacity. Maximal respiration was 46% greater in females than males (female: 411.3 ± 35.1, male: 281.9 ± 29.7 pmol O2·min-1·μg DNA-1; P = 0.04) and spare respiratory capacity was 89% greater in females than males (female: 259.5 ± 26.4, male: 122.0 ± 23.7; pmol O2·min-1·μg DNA-1; P = 0.03). There was no interaction of gender by maternal diet for the mitochondrial stress variables analyzed [non-mitochondrial respiration, basal respiration, ATP production, proton leak, maximal respiration, and spare respiratory capacity (P ≥ 0.15)]. There was no interaction or main effects of gender or maternal diet on the variables analyzed for glycolytic stress [non-glycolytic acidification, glycolysis, glycolytic capacity, and glycolytic reserve (P ≥ 0.34)]. In conclusion, the mitochondrial respiration of satellite cells differs based on offspring gender but not maternal diet. Increased maximal respiration and spare respiratory capacity may give female lambs a greater ability to meet metabolic challenges and face energetic demands.
See more of: ASAS Graduate Student Poster Competition: MS Division
See more of: ASAS Graduate Student Poster Competition: MS Division
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