1726
Effects of linseed oil and propolis additives on protozoa population in dairy cows
Effects of linseed oil and propolis additives on protozoa population in dairy cows
Tuesday, July 22, 2014
Exhibit Hall AB (Kansas City Convention Center)
Abstract Text: Although the role of protozoa in the rumen fermentation is unquestionable, the truth is that its elimination may represent, in some cases, an additional benefit to the ruminant as increased microbial N outflow. The objective of this experiment was to examine the effect of linseed oil and additives on the population of ciliate protozoa in the rumen of dairy cow. Four lactating Holstein cows were used in a 4X4 Latin square design. Diets were composed of 600:400 forage:concentrate (dry matter basis-DM). The treatments were: basal diet (C); basal diet + linseed oil (25 g.kg-1 DM) (LO); basal diet + linseed oil + propolis (10 mg flavonoids.kg-1 DM) (LOP); basal diet + linseed oil + propolis + vitamin E (375 UI.kg-1 DM) (LOPE). Samples of rumen contents were obtained (2 days in each period) manually through the fistula approximately 30 min before feeding and were fixed by diluting with an equal volume of formalin solution (18.5% formaldehyde final concentration). Total counts and generic distribution of protozoa were made in 100 microscopic fields at a magnification of 100x according to Dehority (1993). Statistical analyses were performed by analysis of variance (P < 0.05) and orthogonal contrasts to compare effects of 1) Linseed oil (C vs. LO, LOP and LOPE), 2) Propolis (LO vs. LOP and LOPE); 3) Vitamin E interaction (LOP vs. LOPE). There were no effects of linseed oil on the species of Entodinium (P = 0.097), Isotricha (P = 0.143) and total protozoa population (P = 0.092). In general, the Endodinium species was represented by the largest proportion (99.69%) and a lower Isotricha species (0.30%). However, the Diplodiniinae subfamily was not identified. It can be conclued that linseed oil and propolis additives do not change protozoa population.
Keywords: Microbiology, Omega-3, Additives
Parameters |
Treatments |
SEM |
Orthogonal contrasts1 |
|||||
C |
LO |
LOP |
LOPE |
1 |
2 |
3 |
||
Nx105 ciliates/mL |
||||||||
Entodinium |
4.894 |
3.731 |
4.097 |
3.026 |
0.403 |
0.097 |
0.813 |
0.226 |
Isotricha |
0.024 |
0.008 |
0.011 |
0.008 |
0.004 |
0.143 |
0.879 |
0.792 |
Total |
4.918 |
3.739 |
4.108 |
3.034 |
0.405 |
0.092 |
0.814 |
0.222 |
% Total |
||||||||
Entodinium |
99.58 |
99.83 |
99.76 |
99.60 |
0.111 |
0.633 |
0.635 |
0.678 |
Isotricha |
0.41 |
0.16 |
0.24 |
0.39 |
0.111 |
0.633 |
0.635 |
0.678 |
1P -value.