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696
The effects of poor maternal nutrition on dam and offspring inflammatory status throughout gestation

Wednesday, July 20, 2016: 9:45 AM
251 F (Salt Palace Convention Center)
Amanda K. Jones , Department of Animal Science, University of Connecticut, Storrs, CT
Sambhu M. Pillai , Department of Animal Science, University of Connecticut, Storrs, CT
Maria L. Hoffman , Department of Animal Science, University of Connecticut, Storrs, CT
Katelyn K. McFadden , Department of Animal Science, University of Connecticut, Storrs, CT
Kristen E. Govoni , Department of Animal Science, University of Connecticut, Storrs, CT
Steven A. Zinn , Department of Animal Science, University of Connecticut, Storrs, CT
Sarah A. Reed , Department of Animal Science, University of Connecticut, Storrs, CT
Audio File Recorded Presentation (mp4)
Abstract Text:

We hypothesized that poor maternal nutrition during gestation exaggerates the inflammatory status of ewes throughout gestation, and this would be reflected in the immune profile of offspring during late gestation and at parturition. Pregnant Western White-faced ewes (n = 78) were individually housed and fed 100% (CON), 60% (RES) or 140% (OVER) of NRC for TDN beginning at d 30.2 ± 0.2 of gestation. Whole blood was collected from a subset of ewes at d 24.0 ± 0.9 and d 135.0 ± 0.3 of gestation (n = 4 ewes per diet per day) and from 3 to 4 offspring per diet euthanized at d 135 of gestation or within 24 h of parturition. Whole blood RNA was isolated and expression of 84 genes mediating inflammation were profiled using a real-time PCR array. Data were analyzed using PROC MIXED in SAS for main effects and interaction of diet and day of gestation for ewes and main effect of maternal diet for offspring with the PDIFF option for mean comparisons. In ewes, regardless of diet, relative to d 24, interleukin (IL) 17β, receptors for IL1, IL6, IL8, IL10α and IL10β, colony stimulating factor (CSF) 2, CSF3, tumor necrosis factor superfamily member (TNFSF)13, TNFSF13β, chemokine ligand 17, chemokine receptor 1, vascular endothelial growth factor A, and platelet factor 4 increased 3.8-, 1.7-, 2.1-, 2.4-, 1.5-, 1.3-, 1.9-, 2.0-, 1.6-, 1.9-, 3.7-, 1.7-, 1.7, and 2.5-fold at d 135 of gestation, respectively (P ≤ 0.05). In contrast, chemokine ligand 10 decreased 4.1-fold at d 135 relative to d 24 in ewes, regardless of diet (P = 0.02). In OVER ewes, TNFSF4 decreased 1.5-fold compared with CON (P ≤ 0.05). Interleukin 1 receptor antagonist (IL1RN) increased 1.8-fold in RES ewes at d 135 compared with CON at d 24 (P ≤ 0.04). In offspring, chemokine ligand 22 increased 2.8-fold in OVER compared with CON at d 135 (P ≤ 0.05). At parturition, interferon γ decreased 3.0- and 3.8-fold in OVER and RES, respectively, compared with CON (P ≤ 0.006). In conclusion, inflammatory progression is characteristic of advancing gestation and the increased expression of IL1RN, an antagonist of IL1α and IL1β, in RES ewes at d 135 may be a protective mechanism suppressing pro-inflammatory signaling. The inflammatory profile of offspring was altered by poor maternal nutrition which may negatively affect growth and health if persistent postnatally, thereby reducing offspring productivity. 

Keywords: inflammation, maternal nutrition, sheep

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