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633
Microbial count, fermentation, and aerobic stability of regular and brown midrib corn hybrids ensiled with or without a combo inoculant at high moisture concentrations

Wednesday, July 20, 2016: 11:15 AM
Grand Ballroom H (Salt Palace Convention Center)
Juan J Romero , Department of Crop Science, North Carolina State University, Raleigh, NC
Jinwoo Park , Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University, Jinju, Korea, The Republic of
Marco A Balseca-Paredes , Department of Crop Science, North Carolina State University, Raleigh, NC
Yuchen Zhao , Department of Animal Nutrition and Feed Science, China Agricultural University, Beijing, China
Young Ho Joo , Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University, Jinju, Korea, The Republic of
Adam Heitman , Department of Crop Science, North Carolina State University, Raleigh, NC
Eduardo Gutierrez-Rodriguez , Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, NC
Miguel S Castillo , Department of Crop Science, North Carolina State University, Raleigh, NC
Abstract Text:

The objective was to evaluate the effects of a combo inoculant on microbial count, fermentation, and aerobic stability of 4 hybrids of corn ensiled at high moisture concentrations. Treatment design was the factorial combination of 4 corn-types ensiled with (INO) and without (CON) inoculant. Corn-types (HYB) were TMF2R737, F2F817 (A and B, respectively; from Mycogen), P2089YHR, and P1449XR (C and D, from Pioneer). B and D were brown midrib hybrids. The inoculant added contained Lactobacillus buchneri and Pediococcus pentosaceus (4 × 105 and 1 × 105 cfu/g of fresh corn). Experimental design was a complete randomized design with 6 replicates (silos). Corn was chopped, treated or not with inoculant, packed into 7.6L bucket silos, and stored for 100 d. At d 0 there were differences due to hybrids solely (P ≤ 0.05). The %DM was 30.5, 26.3, 31.1, and 31.5 for A, B, C, and D, respectively; lactic acid bacteria (LAB) count (log cfu/g of fresh corn) was similar for C and D (8.4) and both were greater than A and B (7.7±0.23); yeast count was greatest in C (6.8 vs. 6.0±0.14), and mold count was lowest in D vs. the others (4.6 vs. 5.2±0.15; P ≤ 0.05). At opening, INO increased (P ≤ 0.05) LAB (9.3 vs. 7.1±0.29), acetic acid (3.44 vs. 1.32±0.35% of DM), 1,2-propanediol for all HYB (0.7 vs. 0.0) except D (0.3±0.11% of DM; INO × HYB, P < 0.01); decreased yeast (3.1 vs. 4.6±0.45) and molds (1.5 vs. 3.0±0.61); and extended the aerobic stability (582 vs. 111±128 h) vs. CON. However, INO decreased (P ≤ 0.05) DM recovery (95.6 vs. 97.4±1.05%), lactic acid for all HYB (4.2 vs. 7.6) except A (4.9±0.59% of DM; INO × HYB, P < 0.01), and WSC (1.2 vs. 2.3±0.17% of DM) vs. CON. Also, INO increased pH for all HYB (4.1 vs. 3.9) except D (3.9±0.03, INO × HYB, P < 0.01) and decreased ethanol only for D (0.8 vs. 1.3) but not for the others (0.6±0.08% of DM, INO × HYB, P < 0.01). The results indicate that the inoculant used consistently improved aerobic stability across HYB by increasing acetic acid and reducing fungal counts but reduced DM recovery of the corn ensiled at high moisture.

Keywords:

Corn Silage
Microbial counts
Combo inoculant