The different effects of ferrous glycine chelate and ferrous sulfate to intestinal porcine epithelial cells

Abstract Text:

This study was conducted to investigate the effects of ferrous glycine chelate and ferrous sulfate on cell proliferation and gene expression of iron related transporters in intestinal porcine epithelial cells (IPEC-J2). When IPEC-J2 cells covered 80%~90% of the petri dish, it were treated with different concentration of FeSO₄ and Fe-Gly (0, 50, 100, 200μmol/L as the low concentration; 16000, 32000, 64000, 128000 as the high concentration) for 12h and 24h to determine the cell survival rate. Besides, IPEC-J2 cells were also treated with FeSO₄ and Fe-Gly (50 μmol/L as iron) for 2h, and then quantitative Real-time PCR was applied to detect the mRNA expression of DMT1, FPN1, Dcytb and PepT1. The results showed that both FeSO₄ and Fe-Gly nearly have no toxicity to cells in low concentration, however, high concentration of iron solution could significantly affect cell survival. The influence on cell viability caused by FeSO₄ was more obvious in high concentration compared with Fe-Gly. The qRT-PCR results revealed that Fe-Gly had significant lower expression of DMT1, FPN1 and Dcytb than FeSO4 (P<0.05), while there was no difference on the expression of PepT1. For the physiological function of Dcytb, DMT1 and FPN1 are related with iron ions transportation, it reminded us that FeSO₄may own more free iron ion than Fe-Gly in the same concentration. Fe-Gly had a better stabilization than FeSO4, which means it can prevent iron toxicity to cell when in a high concentration. 

Keywords:

Ferrous glycine chelate; Ferrous sulfate; IPEC-J2; Cell proliferation; Gene expression