Some abstracts do not have video files because ASAS was denied recording rights.
466
The effect of SNPs in the promoter on expression of CYP2E1 gene and boar taint
Effect of SNPs in the Promoter on Expression of CYP2E1 Gene and Boar Taint.
H. Archer1; M. Jafarikia2; B. Lillie3; F. Schenkel2; and E. J. Squires1.
Department of Animal Bioscience, University of Guelph1, Center for Genetic Improvement of Livestock, University of Guelph2, Department of Pathobiology, Ontario Veterinary College3
Boar taint, an unfavourable odour detected in the meat of intact male pigs, is caused by the accumulation of two compounds; androstenone and skatole. Despite mounting welfare concerns, surgical castration of all male piglets is still the most common control method in production systems. The need for new methods to control boar taint is therefore a necessity. Genetic selection represents one such alternative. Among the genes known to be involved in boar taint metabolism, CYP2E1 has repeatedly proven influential. The aim of this study was to identify SNPs within the CYP2E1 promoter affecting gene expression and boar taint. Genotypes were obtained from a previously developed list of 7 single nucleotide polymorphisms (SNPs) on 66 boars from three major swine breeds; Duroc, Landrace and Yorkshire. RNA was isolated from liver tissue and quantitative PCR was performed to measure CYP2E1 gene expression. Association analysis was run correlating genotype and CYP2E1 expression (ΔCT) using PROC GLM (SAS Version 9.4). Weight was included as a fixed effect. The effect of breed, androstenone and skatole concentrations on CYP2E1 expression were also tested. All SNPs had a MAF > 0.05. Results indicated that 1 SNP within the CYP2E1 promoter was significantly associated with CYP2E1 expression at α < 0.05. An additional 3 SNPs demonstrated association at α < 0.10. While weight was significantly associated with gene expression, breed was found to have no effect. Significant within breed variation in CYP2E1 expression was observed, indicating significant differences in gene expression among individuals. Androstenone and skatole were significantly associated, with means of 1.33 ug/g and 0.58 ug/g, respectively. Though SNPs were significantly associated with gene expression, no associations were observed between gene expression and androstenone or skatole in fat. Due to this lack of association between expression and boar taint, results indicate that CYP2E1 mRNA expression alone is not a key indicator for boar taint.
Keywords:
Boar taint, CYP2E1, SNP