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Content of Soluble Non-Starch Polysaccharides β-Mannan and Xylan in Legume Meals, Non-Legume Meals, and Cereal Grains or Cereal Grain by-products

Tuesday, March 18, 2014
Grand Ballroom - Posters (Community Choice Credit Union Convention Center)
Jon E Ferrel , Elanco Animal Health, Greenfield, IN
David M Anderson , Elanco Animal Health, Greenfield, IN
Humg-Yu Hsiao , Elanco Animal Health, Greenfield, IN
Abstract Text:

Feedstuffs for swine nutrition contain a variety of anti-nutritive non-starch polysaccharides (NSP) including β-mannan (BGM) and xylan (XYL) which range in content amongst ingredients. An experiment was conducted to estimate total and water soluble content (as is basis) of BGM and XYL in 5 legume, 11 non-legume meals and 11 cereal grains and cereal grain by-products. A ground sample (sieved, 40 mesh screen) of each was suspended in 80% methanol solution and shaken overnight at 40°C. Filtered samples were washed with 80% methanol, ethanol, hexane and air dried. Samples were suspended with phosphate buffer (20 mM, pH = 7.0) and boiled for one hour. Upper portion of centrifuged samples were hydrolyzed by 1.0 M sulfuric acid for GC analysis to determine soluble content. The remaining portion was hydrolyzed by 1.0 M sulfuric acid for GC analysis and corrected for weight to estimate total content.  Legume protein meal total and soluble content of BGM ranged from 0.36 to 1.60% (SD=0.49) and 0.30 to 1.45% (SD=0.49), respectively.  XYL total and soluble content ranged from 1.00 to 3.10% (SD=0.82) and 0.22 to 0.60% (SD=0.16), respectively. Non-legume protein meal BGM total and soluble content ranged from 0.10 to 39% (SD=14.36) and 0.026 to 7.9% (SD=2.45), respectively. XYL total and soluble content ranged from 0.59 to 11.90% (SD=4.18) and 0 to 1.2% (SD=0.37), respectively.  Grain and grain by-product total and soluble BGM content ranged from 0 to 1.0% (SD=0.274) and 0 to 0.21% (SD=0.07), respectively.  XYL total and soluble content ranged from 1.75 to 16% (SD=4.89) and 0.08 to 3.10% (SD=1.08), respectively. A better knowledge of soluble BGM and XYL concentration of feedstuffs may provide an opportunity to improve pig performance and reduce their anti-nutritive impact.

Selected Ingredient

β-mannan content,%

Xylan content,%

Water soluble

Total

Water soluble

Total

SBM, 48%

1.1

1.2

0.22

1.4

Sesame meal

1.95

4.93

0.5

0.89

Rapeseed meal

0.39

0.68

0.3

1.3

Guar meal, 40%CP

7.9

9.27

0.18

4.1

Sunflower meal

0.36

0.77

0.06

3.2

Palm Kernel Meal

3

39

0

1.5

Corn

0.03

0.05

0.18

2.62

Corn Germ Meal

0.03

0.15

1.2

10.9

Wheat, grain

0.21

0.26

0.6

4.5

Wheat midds

0.05

0.35

2.64

11.3

Barley

0.02

0.27

0.75

4.5

Sorghum

0

0

0.08

2.5

Keywords: NSP, mannan, xylan