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Evaluating the inclusion level of medium chain fatty acids to reduce the risk of PEDV in feed and spray-dried animal plasma
Research has confirmed that chemical treatments, such as medium chain fatty acids (MCFA) and commercial formaldehyde, can be effective to reduce the risk of porcine epidemic diarrhea virus (PEDV) cross-contamination in feed. However, the efficacy of MCFA levels below 2% inclusion is unknown. The objective of this experiment was to evaluate if a 1% inclusion of MCFA is as effective at PEDV mitigation as a 2% inclusion or formaldehyde in swine feed and spray-dried animal plasma (SDAP). Treatments were arranged in a 4×2×6 plus 2 factorial with 5 chemical treatments: 1) PEDV positive with no chemical treatment, 2) 0.325% commercial formaldehyde, 3) 1% MCFA, and 4) 2% MCFA; 2 matrices: 1) complete swine diet and 2) SDAP; 6 analysis days: 0, 1, 3, 7, 14, and 21 post inoculation; and 1treatment each of PEDV negative untreated feed and plasma. Matrices were first chemically treated, then inoculated with PEDV, stored at room temperature until being analyzed by RT-qPCR. Data were analyzed by the GLIMMIX procedure of SAS. The analyzed values represent threshold cycle (CT), at which a higher CT value represents less detectable RNA. All main effects and interactions except for day×form were significant (P<0.02). Feed treated with MCFA, regardless of inclusion level, had fewer (P<0.05) viral particles than feed treated with formaldehyde. However, the SDAP-treated with either 1% or 2% MCFA had similar (P > 0.05) concentrations of detectable PEDV RNA as the untreated SDAP, while the SDAP treated with formaldehyde had fewer viral particles (P<0.05). The complete feed had a lower (P<0.05) quantity of PEDV RNA than SDAP (34.2 vs. 32.2 for feed vs. SDAP, respectively) (P<0.05). Analysis day also decreased (P<0.05) the quantity of detectable viral particles from d 0 to d 21, (33.2 vs. 39.0, respectively). In summary, time, formaldehyde, and MCFA all appear enhance RNA degradation of PEDV in swine feed and ingredients, but their effectiveness varies within matrix. The 1% inclusion level of MCFA was as effective as 2% in complete feed, but neither were effective at reducing the magnitude of PEDV RNA in SDAP.
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Chemical Treatment |
|
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Matrix |
Negative |
Positive |
MCFA 1% |
MCFA 2% |
Formaldehyde |
Pooled SEM |
Feed |
undetectable |
34.2 |
41.4 |
42.8 |
37.2 |
0.98 |
SDAP |
undetectable |
32.2 |
32.4 |
31.6 |
37.6 |
|
Keywords: PEDV, medium chain fatty acids, feed matrix