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Effects of Mannan Oligosaccharides and Lactobacillus Mucosae on the Intestinal Morphology of Weanling Pigs Challenged with Escherichia coli Lipopolysaccharides
Tuesday, March 14, 2017
Grand Ballroom Foyer (Century Link Center)
Yanshuo S Li
,
University of Nebraska, Lincoln, NE
Melanie D. Trenhaile
,
University of Nebraska, Lincoln, NE
Dana M. van Sambeek
,
University of Nebraska, Lincoln, NE
Kelly C Moore
,
University of Nebraska, Lincoln, NE
Shana M Barnett
,
University of Nebraska, Lincoln, NE
Samodha C. Fernando
,
University of Nebraska, Lincoln, NE
Thomas E. Burkey
,
University of Nebraska-Lincoln, Lincoln, NE
Phillip S. Miller
,
University of Nebraska-Lincoln, Lincoln, NE
To determine the effects of feeding mannan oligosaccharides (MOS) and
Lactobacillus mucosae (LM) on intestinal morphology of weanling pigs under immune challenge, 96 pigs (BW = 5.88 kg; d 23 post-farrowing) were randomly allotted to 16 experimental pens with a 2 × 2 factorial arrangement of treatments (4 pens per treatment; mixed gender). Diets with or without 0.1% MOS were randomly assigned to pens and 10
9 cfu/pig LM broth or an equal volume of control broth were top-dressed daily. Pigs were given 1 of the 4 dietary treatments (control, MOS, LM, and MOS+LM) during phase-1 and phase-2 (d 0 to 7 and d 7 to 21 post-weaning, respectively). On d 14, all pigs were challenged with 100 μg/kg BW of
Escherichia coli lipopolysaccharides (LPS). On d 15 and 21, 1 pig per pen was euthanized for collection of intestinal tissue samples. Villus height (VH), crypt depth (CD), and villus surface area of duodenal and ileal samples were measured. Data were analyzed using PROC GLIMMIX of SAS. On d 15, feeding LM tended (
P < 0.10) to decrease ileal CD; pigs fed LM had decreased (
P < 0.05) ileal CD compared to pigs fed the control diet, but were not different (
P > 0.10) from the pigs fed MOS or MOS+LM diets. However, all other ileal and duodenal measurements on d 15 and 21 were not different (
P > 0.10) among treatments. In conclusion, feeding LM may have beneficial effects on gut health of weanling pigs under LPS-challenge.
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P-values
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Items, μm
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Control
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MOS
|
LM
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MOS+LM
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SEM
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MOS
|
LM
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MOS*LM
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d 15 duodenum
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VH
|
322
|
344
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368
|
340
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35
|
0.929
|
0.565
|
0.483
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CD
|
360
|
319
|
374
|
372
|
34
|
0.530
|
0.342
|
0.573
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Villus area, μm2
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36,350
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41,504
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43,979
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39,543
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5,962
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0.953
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0.643
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0.437
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d 15 ileum
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VH
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329
|
312
|
354
|
290
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32
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0.225
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0.968
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0.474
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CD
|
288b
|
267
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246a
|
259
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14
|
0.773
|
0.091
|
0.239
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Villus area, μm2
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38,023
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36,240
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42,575
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32,523
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5,330
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0.289
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0.939
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0.453
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d 21 duodenum
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VH
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493
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574
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522
|
544
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35
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0.163
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0.991
|
0.411
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CD
|
479
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469
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461
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425
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30
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0.465
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0.321
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0.669
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Villus area, μm2
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75,077
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92,409
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81,651
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82,906
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7,212
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0.222
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0.843
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0.287
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d 21 ileum
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|
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VH
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469
|
427
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482
|
445
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28
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0.191
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0.603
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0.938
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CD
|
303
|
285
|
273
|
289
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23
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0.976
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0.587
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0.465
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Villus area, μm2
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62,241
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56,517
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62,216
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55,510
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5,329
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0.266
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0.925
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0.928
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a-bMeans with a different superscript letter differ (P < 0.05).