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A protective effect of IGF-activated plasma protein (CTCgrow) on lipopolysaccharide-induced intestinal dystrophy in rats
In livestock industry, dietary plasma protein has shown the improved growth performance of weaned animals and it seems to be more than nutrient content itself. The exact mechanism of plasma protein on enhancing growth performance is not fully understood yet, but dietary insulin-like growth factors (IGFs) in plasma protein might have an important role. But it has been also suggested that IGFs with binding protein might attenuate the growth-promoting effects of IGFs. Therefore, we conducted an experiment to determine the protective effects of commercially available IGF-activated plasma protein product (by de-binding with protein, CTCgrow) on intestinal dystrophy induced by lipopolysaccharides (LPS).
Forty-two rats (4 week-old) were allotted into six treatments in a 2x3 factorial design. One factor was dietary supplementation (CON, control; PP, 50 g/kg of plasma protein; and aIGFPP, 3.4 g/kg of IGF-activated plasma protein) and the other factor was LPS-challenge (PBS or LPS). Basal diet was formulated based on NIH-31 diet. During 4 week of feeding period, rats were allowed to the diets ad libitum and growth performance was recorded weekly. On day 28, rats were injected with either LPS or PBS and growth performance was recorded every day for 3 days. At day 3 post-injection, rats were sacrificed and gut morphological change was investigated in jejunum samples.
Before LPS-challenge, body weight and feed intake of aIGFPP group were numerically higher than those of other groups. After LPS-challenge, aIGFPP alleviated the weight loss induced by LPS-challenge and showed significantly higher feed efficiency than PP (P < 0.05). In non-challenged groups, there were significantly higher villus height (VH) and VH:CD ratio and lower crypt depth (CD) in aIGFPP than other groups (P < 0.05). And the number of goblet cells per villus was also significantly higher in aIGFPP than other groups (P < 0.05). And there was a tendency that LPS-challenged groups showed lower VH and higher CD than non-challenged groups in respective dietary treatment groups. In LPS-challenged groups, VH and VH:CD ratio were numerically higher in CON and aIGFPP than PP. The number of goblet cells per villus was significantly higher in LPS-challenged aIGFPP than other LPS-challenged groups (P < 0.05).
These results indicated that aIGFPP had a growth-promoting effect by improvement of intestinal morphology before LPS-challenge. Also aIGFPP demonstrated a protective effect on gut mucosal injury induced by LPS.
Keywords: Rats, Intestine, Gut morphology, IGF, Plasma protein